ORIGINAL ARTICLE

Effect of Caesalpinia pulcherrima (L.) Sw. seeds on serum glucose and other metabolic parameters of normal and alloxan - induced diabetic rats

M Balasundaram, Arumugam Dhanesh Gandhi, Purusothaman Kavitha, Ranganathan Babujanarthanam

M Balasundaram
Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore-632115, Tamil Nadu, India

Arumugam Dhanesh Gandhi
Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore-632115, Tamil Nadu, India

Purusothaman Kavitha
Department of Biochemistry, KMG College of Arts and Science, Gudiyattam Tamil Nadu, India

Ranganathan Babujanarthanam
Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore-632115, Tamil Nadu, India. Email: babukmg@gmail.com
Online First: October 15, 2018 | Cite this Article
Balasundaram, M., Dhanesh Gandhi, A., Kavitha, P., Babujanarthanam, R. 2018. Effect of Caesalpinia pulcherrima (L.) Sw. seeds on serum glucose and other metabolic parameters of normal and alloxan - induced diabetic rats. Diabesity 4(4). DOI:10.15562/diabesity.2018.49


Oral administration of the ethanol extract of Caesalpinia pulcherrima seeds (CP - 250 and 500 mg/kg) caused significant fall in blood glucose levels even at 2½ h after a single dose of treatment in normal fasted and glucose loaded Wistar rats. At 250 mg/kg dose level, CP completely prevented the elevation of blood glucose caused by oral glucose feeding. In alloxan diabetic rats, CP was able to lower the blood glucose level to around 132 mg / 100 ml from 10th day and thereafter. The biochemical findings were supported by histopathological studies of liver, kidney and pancreas of control and treated rats. CP was able to increase catalase levels of diabetic rats. Reduced levels of serum protein and elevated levels of serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase(SGPT), alkaline phosphatase(ALP), cholesterol, triglycerides, creatinine and uric acid were almost normalised in CP treated diabetic rats. CP was also able to reduce in vitro lipid peroxidation in rat liver microsomes and inhibit 1- diphenyl – 2-picryl hydrazyl (DPPH) induced free radicals significantly.

References

Momin A, Role of Indigenous Medicine in Primary Health Care. Proceedings of First International Seminar on Unani Medicine, (New Delhi ) 1987, 54.

Shukla R, Sharma S B, Puri D, Prabhu K M & Murthy P S, Medicinal plants for treatment of diabetes mellitus, Indian J Clin Biochem, 15 (2000) 169.

Tiwari A K & Rao M, Diabetes mellitus and multiple therapeutic approaches of phytochemicals: present status and future prospects, Curr Sci, 83 (2002) 30.

Waynforth H B, Experimental and Surgical Techniques in the Rat,( Academic Press,London ) 1980, 160.

Babu V, Gangadevi T & Subramoniam A, Anti-hyperglycaemic activity of Cassia kleinii leaf extract in glucose fed normal rats and alloxan induced diabetic rats, Indian J Pharmacol, 34 (2002) 409.

Reitman S & Frankel S, Determination of serum glutamate oxaloacetate and glutamic pyruvic acid transaminases, Amer J Clin Pathol, 28 (1957) 56.

Kind P R N & King E J, Estimation of plasma phosphatase by determination of hydrolysed phenol with amino anti pyrine, J Clin Pathol, 7 (1954) 322 .

Zak B, Boyle A J & Zlatkis A, A method for the determination of cholesterol, J Clin Med,41(1953)48.

Lowry H D, Rosenberg N J, Farr A L & Randall R S, Protein measurement with Folin’s phenol reagent, J Biol Chem, 193 (1951) 265.

Bowers L D, Kinetic serum creatinine assays I. The role of various factors in determining specificity, Clin Chem 26, (1980) 551.

Eichhom E, Zalma waki S, Rotenburg E A & Fanis B,Uric acid estimation in serum and urine, J Clin Pathol, 14, ( 1961 ) 450. 12

Muller P H , Schmulling RM, Liebich H & Eggstein M, A fully enzymatic triglyceride determination, J Clin Chem 15 (1977 ) 457.

Yoshiyuki K, Michinori K, Tadoto T, Shigree A & Hiromichi O, Studies on Scutelariae radix, Effect of lipid peroxidation of rat liver, Chem Pharm Bull, 29 (1981) 2610.

Masao H, Yang X W, Migashiro H & Namba T, Inhibitory effects of monomeric and dimeric phenyl propanoids on lipid peroxidation in vivo and in vitro,Phytother Res, 7 (1993) 395.

Sreejayan N & Rao M N A, Free radical scavenging activity by curcuminiods.Drug Res, 46 (1996) 169.

Prasanth Kumar V, Shasidhara S,Kumar M M & Sridhara B Y,Effect of Luffa echinata on lipid peroxidation and free radical scavenging activity, J Pharm. Pharmacol, 52 (2000) 891.

Snedecor G W & Cochran W G, Statistical Methods (Iowa State University Press, Iowa, USA) 1980, 75.

Wild S, Roglic G, Green A, Sicre R & King H, Global prevalence of diabetes estimates for the year 2000 and projections for 2030, Diabetes Care, 27 (2004) 1047.

Nagarajan S, Jain H C &Aulakh G S, Indigenous Plants Used in the Control of Diabetes.( Council of Scientific and Industrial Research, New Delhi)1987,588.

Twaij H A A & Al-Badr,Hypoglycaemic activity of Artemisia herba alba,J Ethnopharmacol, 24 (1988) 123.

Gupta S S, Prospects and perspectives of natural plant products in medicine, Indian J Pharmacol, 26 (1994) 5.

Ansari Z & Nehal M, Hypoglycaemic effect of Momordica charantia seed mediates through extrapancreatic actions, J Sci Pharm, 4 (2003) 65.

Mukherjee P K, Saha K, Pal M & Saha B P, Effect of Nelumbo nucifera rhizome extract on blood sugar levels in rats, J Ethnopharmacol, 58 (1997) 207.

Sharma M K, Khara A K & Feroz H, Effect of neem oil on blood glucose levels of normal, hyperglycaemic and diabetic animals, Indian Med Gaz, 117 (1983) 380.

Dhanabal S P, Kokati C K, Ramanathan M, Kumar E P & Suresh B, Hypoglycaemic activity of Pterocarpus marsupium Roxb, Phytother Res 20 (2006) 4.

Dhawan D, Bandhu H K, Singh B, Singh & Nagpal J P, Effect of Securigera securidaca on blood glucose levels of normal and alloxan induced diabetic rats, Pharmaceut Biol, 39 (1996) 62.

Ohiri F C, Esimone C O, Nwafor S V, Okoli C O & Ndu O O, Hypoglycaemic properties of Viscum album (Mistletoe) in alloxan induced diabetic animals, Pharmaceut Biol, 41 (2003) 184.

Kameswararao B, Renuka S P, Rajasekhar M D, Nagaraju N & Apparao C H, Antidiabetic activity of Terminalia pallida fruit in alloxan- induced diabetic rats, J Ethnopharmacol, 85 (2002) 169.


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