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Evaluation of Antidiabetic and Antioxidative Efficacy of Strychnos Potatorum (Nirmali) Seeds Extract in High Fat Diet Fed- Low Dose Streptozotocin Induced Experimental Type 2 Diabetes in Rats


Objective: Type 2 diabetes mellitus is a multifactorial, multisystemic chronic endocrine disorder characterized by insufficient insulin secretion in association with insulin resistance. Strychnos potatorum Linn. seeds have been used in the traditional system of medicine for the treatment of various non communicable diseases including diabetes mellitus. In the present study, we have evaluated the antidiabetic and antioxidant property of Strychnos potatorum seeds extract in type 2 diabetic rats. Chronic hyperglycemia promotes auto-oxidation of glucose to generate excessive free radicals which result in the development of both macro- and microvascular complications.

Design: S. potatorum seeds were delipidated and the ethanolic extract was subjected to qualitative phytochemical screening. The total phenolic, flavonoid, carbohydrate and protein contents were estimated. High fat fed and -Low dose streptozotocin induced type 2 diabetic rats were orally treated with S. potatorum seeds extract (500mg/kg b.w./rat/day) for 30 days. The levels of important biochemical indices and oxidative stress markers such as lipid peroxides, hydroperoxides and protein carbonyls were determined in plasma and vital tissues. The levels of both enzymatic and non enzymatic antioxidants were also evaluated.

Results: Diabetic rats showed significantly increased levels of fasting blood glucose and glycosylated hemoglobulin. The oxidative stress markers were significantly increased and levels of both enzymatic and non enzymatic antioxidants were declined. Oral treatment of S. potatorum seeds extract results in the maintenance of normoglycemia by decreasing the levels of oxidative stress markers and improved the status of antioxidants in diabetic rats.

Conclusions: S. potatorum seeds possess significant antidiabetic properties which may partially responsible for its antioxidant properties.


  1. Shaw JE, Sicree RA and Zimmet PZ. Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Research and Clinical Practice. 2010;87(1):4–14
  2. Fatmah AM, Siti BB, Zariyantey AH, Nasar A, Jamaludin M. The Role of Oxidative Stress and Antioxidants in Diabetic Complications. Sultan Qaboos Univ Med J. 2012; 12(1): 5–18.
  3. Ergul Belge Kurutas. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutr J. 2016; 15(71):1-22.
  4. Rena, G.; Hardie, D.G.; Pearson, E.R. The mechanisms of action of metformin. Diabetologia 2017, 60, 1577–1585.
  5. Sharmila C, Subramanian SP. Studies on the Defluoridization Competency of a Mixture of Raw Vermiculite and Strychnos Potatorum linn. Seeds. Der Pharma Chemica 2019, 11(6): 1-6.
  6. Biswas A, Kanti GT, Ghosh, Joydip PA, Kallol B, Halder D. Hypoglycemic Effect of Strychnos Potatorum Linn were Compared with Glipizide on Male Diabetic Rats. Indian Medical Gazette. 2014; 297-303.
  7. Harborne JB.Phytochemical methods, Chapman and Hall Int., New York,1998; Third Edition
  8. Suman RK, Mohanty IR, Borde MK, Maheshwari U, Deshmukh YA. Development of an experimental model of diabetes co-existing with metabolic syndrome in rats. Advances in Pharmacological Sciences. 2016; 1-11
  9. Rakieten N, Rakieten ML, Nadkarni MV. Studies on the diabetogenic action of streptozotocin (NSC-37917).Cancer Chemother Rep 1963;29: 91-98.
  10. Yagi K.A simple fluorometric assay for lipoperoxide in blood plasma.Biochem Med.1976; 15(2):212-6.
  11. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351-8.
  12. Jiang ZY, Hunt JV, Wolff SP. Ferrous ion oxidation in the presence of xylenol orange for detection of lipid hydroperoxide in low density lipoprotein.Anal Biochem. 1992;202(2):384-9.
  13. Misra HP and Fridovich I.The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase.J Biol Chem. 1972;247(10):3170-5.
  14. Takahara S, Hamilton HB, Neel JV, Kobara TY, Ogura Y, Nishimura ET. Hypocatalasemia: a new genetic carrier state. J Clin Invest. 1960; 39:610-9.
  15. Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: biochemical role as a component of glutathione peroxidase. Science. 1973;179(4073):588-90.
  16. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases.The first enzymatic step in mercapturic acid formation.J Biol Chem. 1974;249(22):7130-9.
  17. Omaye ST, Turnbull JD, Sauberlich HE. Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids.Methods Enzymol. 1979;62:3-11.
  18. Desai ID. Vitamin E analysis methods for animal tissues.Methods Enzymol. 1984;105:138-47.
  19. Ravin HA.An improved colorimetric enzymatic assay of ceruloplasmin.J Lab Clin Med. 1961;58:161-8.
  20. Sedlak J and Lindsay RH.Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent.Analytical Biochemistry. 1968;25: 192-205
  21. Bhushan MS, Amita V, Madhavan V. Preclinical valuation of anti-hyperglycemic and antioxidant action of Nirmali (Strychnos potatorum) seeds in streptozotocin-nicotinamide-induced diabetic Wistar rats: A histopathological investigation. Biomarkers and Genomic medicine, 2013; 5:157-163.
  22. Mallikharjuna PB, Rajanna LN, Seetharam YN, et al. Phytochemical studies of Strychnos potatorum L.f a medicinal plant. e-J Chem. 2007;4:510-518.
  23. Shariq I. Sherwani, Haseeb A. Khan, Aishah Ekhzaimy, Afshan Masood, Meena K. Sakharkar. Significance of HbA1c Test in Diagnosis and Prognosis of Diabetic Patients. Biomark Insights. 2016; 11: 95–104.
  24. Singh AK, Dhar DN. Studies on the chemical constituents of the seeds of Strychnos Potatorum L. part I. Planta Med 1977; 32(8):362-367
  25. Sanmugapriya E & Venkataraman S. Anti-inflammatory Effect of Strychnos potatorum. Seeds on Acute and Subacute Inflammation in Experimental Rat Models. Pharmaceutical Biology. 2007; 45(6):435–439.

How to Cite

Subramanian, S. P. (2020). Evaluation of Antidiabetic and Antioxidative Efficacy of Strychnos Potatorum (Nirmali) Seeds Extract in High Fat Diet Fed- Low Dose Streptozotocin Induced Experimental Type 2 Diabetes in Rats. Diabesity, 6(1), 1–8.


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Sorimuthu Pillai Subramanian
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Diabesity Journal